ABSTRACT
Objective@#To investigate the effect of allogeneic red blood cell transfusion on T lymphocyte subsets and natural killer (NK) cells in peripheral blood of patients with gastric cancer during perioperative period.@*Methods@#50 patients with gastric cancer in our hospital from January 2017 to December 2017 were randomly divided into control group (n=25) and observation group (n=25). Perioperative allogeneic erythrocyte transfusion was performed in the observation group, while no allogeneic erythrocyte transfusion was performed in the control group. Blood samples were taken from two groups before and after operation 3 d, and serum T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+ /CD8+ ) and NK cell levels were measured. T cell subsets and NK cell levels of patients in observation group, control group and observation group at different clinical stages before and after operation were counted. According to blood transfusion volume, the patients in the observation group were divided into <4 U group and <4 U group. T cell subsets and NK cell levels before and after operation were compared between the two groups.@*Results@#There was no significant difference in CD3+, CD4+, CD8+, CD4+ /CD8+, NK cells between the two groups before operation (P>0.05). The levels of CD3+, CD4+, CD4+ /CD8+, NK cells in the two groups after operation were lower than those before operation and CD8+ levels were higher than those before operation. The levels of CD3+, CD4+, CD4+ /CD8+, NK cells in the observation group were lower than those in the control group, and the levels of CD8+ in the observation group were higher than those in the control group (P<0.05). The levels of CD3+, CD4+, CD8+, CD4+ /CD8+, NK cells in patients with stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ had no significant difference before operation (P>0.05). After operation, the levels of CD3+, CD4+, CD4+ /CD8+, NK cells in the two groups were lower and CD8+ levels were higher than those before operation. The levels of CD3+, CD4+, CD4+ /CD8+, NK cells in stage Ⅰ-Ⅱ patients were higher than those in stage Ⅲ-Ⅳ patients, and CD8+ levels were lower than those in stage Ⅲ-Ⅳ patients (P< 0.05); There was no significant difference in the levels of CD3+, CD4+, CD8+, CD4+ /CD8+, NK cells between the <4 U group and <4 U group before operation (P>0.05). After operation, the levels of CD8+ in the two groups were higher than those before operation, while the levels of CD3+, CD4+, CD4+ /CD8+, NK cells were lower than those before operation; The levels of CD3+, CD4+, CD4+ /CD8+, NK cells in the <4 U group were higher than those in >4 U group, and CD8+ levels were lower than those in >4 U group (P<0.05).@*Conclusions@#Perioperative allogeneic red blood cell transfusion can affect T cell subsets and NK cell content in peripheral blood of patients with gastric cancer, and there are some differences in the effects of different clinical stages of gastric cancer patients, which can cause the suppression of cellular immune function.
ABSTRACT
Objective To investigate the effect of allogeneic red blood cell transfusion on T lymphocyte subsets and natural killer (NK) cells in peripheral blood of patients with gastric cancer during perioperative period.Methods 50 patients with gastric cancer in our hospital from January 2017 to December 2017 were randomly divided into control group (n =25) and observation group (n =25).Perioperative allogeneic erythrocyte transfusion was performed in the observation group,while no allogeneic erythrocyte transfusion was performed in the control group.Blood samples were taken from two groups before and after operation 3 d,and serum T lymphocyte subsets (CD3 +,CD4 +,CD8 +,CD4 +/CD8 +) and NK cell levels were measured.T cell subsets and NK cell levels of patients in observation group,control group and observation group at different clinical stages before and after operation were counted.According to blood transfusion volume,the patients in the observation group were divided into ＜ 4 U group and ＜ 4 U group.T cell subsets and NK cell levels before and after operation were compared between the two groups.Results There was no significant difference in CD3+,CD4+,CD8+,CD4+/CD8+,NK cells between the two groups before operation (P ＞ 0.05).The levels of CD3 +,CD4 +,CD4 +/CD8 +,NK cells in the two groups after operation were lower than those before operation and CD8 + levels were higher than those before operation.The levels of CD3 +,CD4 +,CD4 +/CD8 +,NK cells in the observation group were lower than those in the control group,and the levels of CD8 + in the observation group were higher than those in the control group (P ＜0.05).The levels of CD3 +,CD4 +,CD8 +,CD4 +/CD8 +,NK cells in patients with stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ had no significant difference before operation (P ＞0.05).After operation,the levels of CD3 +,CD4 +,CD4 +/CD8 +,NK cells in the two groups were lower and CD8 + levels were higher than those before operation.The levels of CD3 +,CD4 +,CD4 +/CD8 +,NK cells in stage Ⅰ-Ⅱ patients were higher than those in stage Ⅲ-Ⅳ patients,and CD8 + levels were lower than those in stage Ⅲl-Ⅳ patients (P ＜ 0.05);There was no significant difference in the levels of CD3 +,CD4 +,CD8 +,CD4 + /CD8 +,NK cells between the ＜ 4 U group and ＜ 4 U group before operation (P ＞ 0.05).After operation,the levels of CD8 + in the two groups were higher than those before operation,while the levels of CD3 +,CD4 +,CD4 +/CD8 +,NK cells were lower than those before operation;The levels of CD3 +,CD4 +,CD4 +/CD8 +,NK cells in the ＜ 4 U group were higher than those in ＞ 4 U group,and CD8 + levels were lower than those in ＞ 4 U group (P ＜ 0.05).Conclusions Perioperative allogeneic red blood cell transfusion can affect T cell subsets and NK cell content in peripheral blood of patients with gastric cancer,and there are some differences in the effects of different clinical stages of gastric cancer patients,which can cause the suppression of cellular immune function.
ABSTRACT
Objective: To explore the effects of monocrotaline (MCT) on right ventricular function and expression of cardiac canonical transient receptor potential channels (TRPC) subfamily in experimental rats. Methods: The SD male rats were randomly divided into 2 groups:Control group, the rats were normally fed and MCT group, the rats received a single dose injection of MCT 60 mg/kg to induce myocardial hypertrophy. n=10 in each group and all animals were treated for 3 weeks. The right ventricular hemodynamics parameters and right ventricular hypertrophy index (RVHI) were measured, right ventricular myocardium tissue section was observed by HE staining, the mRNA and protein expressions of TRPC subfamily were examined by RT-PCR and Western blot analysis. Results: Compared with Control group, MCT group had increased RVSP, RVHI, RV+dp/dtmax and decreased RV-dp/dtmax, all P Conclusion:Right ventricular hypertrophy could be induced by 3 weeks MCT treatment, it up-regulating the mRNA and protein expressions of TRPC6 which might be involved in the occurrence and development of cardiac hypertrophy in experimental rats.
ABSTRACT
Objective To investigate the feasibility and safety of minimally invasive pereutaneous nephro-lithotomy(MPCNL) in treating impacted upper ureteral calculi. Methods 50 cases with impacted upper ureteral cal-culi treted with MPCNL were analyzed retrospectively. Results All cases were rendered stone free at one session, clearance rate was 100%, 1 case required transfusion,no other major complications was observed. Conclusion MPC-NL was safe and effective in treating impacted upper ureteral calculi with a high clearance rate.